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A2780cis cells
Catalog #:
C0017003
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Disease:
Ovarian carcinoma
Derived from tumor tissue of an untreated patient. This cisplatin-resistant cell line has been developed by chronic exposure of the parent cisplatin-sensitive A2780 cell line (C0017002) to increasing concentrations of cisplatin. A2780cis is cross-resistant to melphalan, adriamycin and irradiation. An increased ability to repair DNA damage as well as cytogenetic abnormalities has been observed. In order to retain resistance cisplatinum has to be added to the media for every passage.
Homo sapiens
Ovary
Epithelial
Adherent
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS + 1 uM Cisplatin
1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C. Cells will attach slowly after resuscitation and take up to 7 days to reach confluency. Recommendation: recover cells in media without cisplatin. Add after subculture of attached cells.
Complete culture medium supplemented with 5% (v/v) DMSO
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 11,13
D5S818: 11
D7S820: 10
THO1: 6
TPOX: 8,10
vWA: 15,16
Origin:
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Modal number: 46
Patient:
Not available
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
I
Sterility:
Pathogens:
DNA Profile (STR):
Related cells by Organ:
References for A2780cis cells:
1. Behrens BC, Hamilton TC, Masuda H, Grotzinger KR, Whang-Peng J, Louie KG, Knutsen T, McKoy WM, Young RC, Ozols RF. Characterization of a cis-diamminedichloroplatinum(II)-resistant human ovarian cancer cell line and its use in evaluation of platinum analogues. Cancer Res. 1987 Jan 15;47(2):414-418. PMID: 3539322
Certificate of Analysis/MSDS is available upon request with lot number
