HCT-8 (HRT-18) cells
Catalog #:
C0009017
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Disease:
Ileocecal colorectal adenocarcinoma
Origin:
This cell line was derived from a 67 year-old male.
Species:
Homo sapiens
Colon
Epithelial
Adherent
2n = 46, pseudodiploid
67 year old male
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% horse serum
Subculture at 1:4; feed cells 3 to 4 times weekly. Culture at 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12, 11.3
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19
If use of this culture results in a scientific publication, it should be cited in the publication as: HCT-8 (AddexBio C0009017)
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References for HCT-8 cells:
1. Tompkins WA, Watrach AM, Schmale JD, Schultz RM, Harris JA. Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J Natl Cancer Inst. 1974 Apr;52(4):1101-1110. PMID: 4826581