DSL-6A/C1 cells

Catalog #:

C0018011

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Disease:

Rat pancreatic carcinoma, induced by azaserine

Origin:

DSL-6A/C1 were derived from a transplantable acinar cell carcinoma (DSL-6) that had been established from a primary carcinoma of the pancreas in an intraperitoneal treatment with azaserine in male Lewis rat. The expression of the ductal cell marker OC.2 was shown with a sub-population expressing ductal marker OV-6, CK-7 and CK-19. DSL-6A/C1 cells are reported to be tumorigenic in Lewis rats and produce solid tumors composed of duct like structures surrounded by dense fibrous tissue. The cell line lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and re-grafting. The cultured DSL-6A/C1 tumor cells initially produced amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture. The DSL-6A/C1 cell line expresses the ductal marker cystic fibrosis transmembrane regulator (CFTR). The cell line lacks CCK-A receptors.

Species:

Rattus norvegicus, rat

Tissue:

Rat pancreas

Morphology:

Polygonal, epithelial

Properties:

Adherent

Medium:

Waymouth's MB 752/1 Medium + 10% FBS

Subculture:

Split sub-confluent cultures (80-90%) 1:3 to 1:4 using ; i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO₂; 37°C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO