WI-38 cells
Catalog #:
T0016037
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Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for details
| Frozen vial | USD420.0 |
Disease:
Normal
Origin:
The WI-38 human diploid cell line was derived by Leonard Hayflick from normal embryonic (3 months gestation) lung tissue.
Species:
Homo sapiens, human
Lung
Fibroblast
Adherent
Normal diploid
Female, Caucasian, 3 months gestation fetus
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.
Complete growth culture medium supplemented with 7.5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
THO1:8,9.3
TPOX: 8
vWA: 19,20
If use of this culture results in a scientific publication, it should be cited in the publication as: WI-38 (AddexBio T0016037)
Tissue:
Morphology:
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DNA Profile (STR):
Citation Format:
References for WI-38 cells:
1. HAYFLICK L, MOORHEAD PS. The serial cultivation of human diploid cell strains. Exp Cell Res. 1961 Dec;25:585-621. PMID: 13905658
