BJAB cells

Catalog #:

C0003016

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Disease:

Burkitt lymphoma

Origin:

Established from the inguinal tumor of an EBV-negative 5-year-old African girl with Burkitt lymphoma at the preterminal stage.

Species:

Homo sapiens

Tissue:

Peripheral blood

Morphology:

Round to polymorphic cells

Properties:

Cells growing in suspension, singly and in clumps

Cytogenic data:

human hypodiploid karyotype; 44(40-46)<2n>XX, -10, -21, der(6)t(6;10)(p22;p12), der(7)t(1;7)(p36;p22), t(11;17)(q24;q24), der(11)t(11;17)(q24;q24)t(11;6)(p15;q11)t(6;5)(q22;?), ider(13)(q10)t(3;13)(q26;q14), der(13)t(10;13)(q11;p11)dup(13)(q11q32)t(13;21)(q32;q22), der(21)t(13;21); no IG/MYC translocation detected

Patient:

African female, 5 yrs of age

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS

Subculture:

Seed cells at approximately 6 x 10⁵ cells/ml; optimal split ratio 1:2 every 2-3 days; culture flask should be kept horizontal. Although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Culture at 5% CO₂; 37°C

Freezing Medium:

Complete culture medium supplemented with 10% (v/v) DMSO