RPMI-8226 cells
Catalog #:
C0003002
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| Frozen vial | USD420.0 |
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Disease:
Plasmacytoma; myeloma
Derived from B lymphocytes
Homo sapiens
Blood
Lymphoblast
Suspension
Unstable karyotype in triploid
Male, 61 yrs of age
RPMI1640 + 10% FBS
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 105 viable cells/ml. Do not allow the cell density to exceed 2 to 3 X 106 cells/ml. Culture cells at 5% CO2, 37°C.
Every 2 to 3 days.
Complete culture medium supplemented with 5% (v/v) DMSO
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Origin:
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Medium Renewal:
Freezing Medium:
Biosafety Level:
Sterility:
Pathogens:
Isoenzymes:
G6PD, A
DNA Profile (STR):
Amelogenin: X,Y;
CSF1PO: 12;
D13S317: 11;
D16S539: 9;
D5S818: 11,13;
D7S820: 9,10;
THO1: 8;
TPOX: 8,11;
vWA: 16,18
Related cells by Organ:
References for RPMI-8226 cells:
1. Genty, V., Dine, G., and Dufer, J. Phenotypical alterations induced by glucocorticoids resistance in RPMI 8226 human myeloma cells. Leuk Res, 28: 307-313, 2004.
2. Genty, V., El-Khoury, V., Liautaud-Roger, F., Dine, G., and Dufer, J. Nuclear chromatin patterns in 3 glucocorticoid-resistant RPMI 8226 human myeloma cell sub-lines: correlations with cell growth and immunological phenotype. Cancer Biol Ther, 4: 832-839, 2005.
3. Hwang, W. Y., Gullo, C. A., Shen, J., Poh, C. K., Tham, S. C., Cow, G., Au, M., Chan, E. W., and Teoh, G. Decoupling of normal CD40/interleukin-4 immunoglobulin heavy chain switch signal leads to genomic instability in SGH-MM5 and RPMI 8226 multiple myeloma cell lines. Leukemia, 20: 715-723, 2006.
4. Iijima, M., Momose, I., and Ikeda, D. Increased ABCB1 expression in TP-110-resistant RPMI-8226 cells. Biosci Biotechnol Biochem, 74: 1913-1919.
5. Lee, K. W., Kim, D. S., and Kwon, H. J. CG sequence- and phosphorothioate backbone modification-dependent activation of the NF-kappaB-responsive gene expression by CpG-oligodeoxynucleotides in human RPMI 8226 B cells. Mol Immunol, 41: 955-964, 2004.
6. Rao, P. S., Ramanadham, M., and Prasad, M. N. Anti-proliferative and cytotoxic effects of Strychnos nux-vomica root extract on human multiple myeloma cell line - RPMI 8226. Food Chem Toxicol, 47: 283-288, 2009.
