HCT-116 cells
Catalog #:
C0009005
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| Frozen vial | USD420.0 |
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Disease:
Colorectal carcinoma
Established from the primary colon carcinoma
Homo sapiens
Colon
Epithelial
Adherent
Tumorigenic; the stemline chromosome number is near diploid
Male, adult
AddexBio-Formulated DMEM (C0003-01) + 1X MEM Non-Essential Amino Acids (NEAA)(Thermo 11140050) + 10% FBS or McCoy's 5a Medium + 10% FBS
1:3 to 1:8 using using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: HCT-116 (AddexBio C0009005)
Origin:
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
Sterility:
Pathogens:
Protein Expression:
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Isoenzymes:
AK-1, 1;
ES-D, 1-2;
G6PD, B;
GLO-I, 1;
PGM1, 1;
PGM3, 1
DNA Profile (STR):
Amelogenin: X,Y;
CSF1PO: 7,10;
D13S317: 10,12;
D16S539: 11,13;
D5S818: 10,11;
D7S820: 11,12;
THO1: 8,9;
TPOX: 8,9;
vWA: 17,22
Citation Format:
Related cells by Organ:
References for HCT-116 cells:
1. Borralho, P. M., Kren, B. T., Castro, R. E., da Silva, I. B., Steer, C. J., and Rodrigues, C. M. MicroRNA-143 reduces viability and increases sensitivity to 5-fluorouracil in HCT116 human colorectal cancer cells. Febs J, 276: 6689-6700, 2009.
2. Lee, S. J., Cho, Y. H., Kim, H., Park, K., Park, S. K., Ha, S. D., Kim, W. J., and Moon, S. K. Inhibitory effects of the ethanol extract of Gleditsia sinensis thorns on human colon cancer HCT116 cells in vitro and in vivo. Oncol Rep, 22: 1505-1512, 2009.
3. Shin, S., Lee, E. M., Cha, H. J., Bae, S., Jung, J. H., Lee, S. M., Yoon, Y., Lee, H., Kim, S., Kim, H., Lee, S. J., Park, I. C., Jin, Y. W., and An, S. MicroRNAs that respond to histone deacetylase inhibitor SAHA and p53 in HCT116 human colon carcinoma cells. Int J Oncol, 35: 1343-1352, 2009.
4. Yan, T., Seo, Y., and Kinsella, T. J. Differential cellular responses to prolonged LDR-IR in MLH1-proficient and MLH1-deficient colorectal cancer HCT116 cells. Clin Cancer Res, 15: 6912-6920, 2009.
5. Ye, Q., Feng, B., Peng, Y. F., Chen, X. H., Cai, Q., Yu, B. Q., Li, L. H., Qiu, M. Y., Liu, B. Y., and Zheng, M. H. Expression of gamma-synuclein in colorectal cancer tissues and its role on colorectal cancer cell line HCT116. World J Gastroenterol, 15: 5035-5043, 2009.
6. Zhu, H., Miao, Z. H., Huang, M., Feng, J. M., Zhang, Z. X., Lu, J. J., Cai, Y. J., Tong, L. J., Xu, Y. F., Qian, X. H., and Ding, J. Naphthalimides induce G(2) arrest through the ATM-activated Chk2-executed pathway in HCT116 cells. Neoplasia, 11: 1226-1234, 2009.
