H460 (NCI-H460) cells
Catalog #:
C0016003
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Disease:
Large cell lung carcinoma
Derived from metastatic pleural effusion
Homo sapiens
Lung
Epithelial
Adherent
This is a hypotriploid human cell line
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
1:3 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: H460 (AddexBio C0016003)
Origin:
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
I
Sterility:
Pathogens:
Isoenzymes:
AK-1, 1;
ES-D, 1;
G6PD, B;
GLO-I, 1-2;
Me-2, 1;
PGM1, 1;
PGM3, 1
DNA Profile (STR):
Amelogenin: X,Y;
CSF1PO: 11,12;
D13S317: 13;
D16S539: 9;
D5S818: 9,10;
D7S820: 9,12;
THO1: 9.3;
TPOX: 8;
vWA: 17
Related cells by Organ:
References for H460 cells:
1. Lim, S. J., Choi, M. K., Kim, M. J., and Kim, J. K. Alpha-tocopheryl succinate potentiates the paclitaxel-induced apoptosis through enforced caspase 8 activation in human H460 lung cancer cells. Exp Mol Med, 41: 737-745, 2009.
2. Pesic, M., Podolski, A., Rakic, L., and Ruzdijic, S. Purine analogs sensitize the multidrug resistant cell line (NCI-H460/R) to doxorubicin and stimulate the cell growth inhibitory effect of verapamil. Invest New Drugs, 28: 482-492.
3. Liu, H., Li, L., Li, X. Q., Liu, X. J., and Zhen, Y. S. Enediyne lidamycin enhances the effect of epidermal growth factor receptor tyrosine kinase inhibitor, gefitinib, in epidermoid carcinoma A431 cells and lung carcinoma H460 cells. Anticancer Drugs, 20: 41-49, 2009.
4. Mollah, M. L., Song, J. C., Park, C. H., Lee, G. D., Hong, J. H., Ryoo, Z. Y., Lee, S., Chang, K. T., and Kim, K. S. Anticancer activity and apoptotic effects of Bulnesia sarmienti against human lung cancer H460 cells. Oncol Res, 18: 259-267, 2009.
5. Borgovan, T., Bellistri, J. P., Slack, K. N., Kopelovich, L., Desai, M., and Joe, A. K. Inhibition of BCL2 expression and activity increases H460 sensitivity to the growth inhibitory effects of polyphenon E. J Exp Ther Oncol, 8: 129-144, 2009.
6. Pesic, M., Andjelkovic, T., Bankovic, J., Markovic, I. D., Rakic, L., and Ruzdijic, S. Sulfinosine enhances doxorubicin efficacy through synergism and by reversing multidrug resistance in the human non-small cell lung carcinoma cell line (NCI-H460/R). Invest New Drugs, 27: 99-110, 2009.
