5 - TMR - RRADDSDDDDD (a CKII substrate)

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Synonyms: RRADDSDDDDD, 5 - TMR labeled

Functional Activity:

RRADDSDDDDD is a selective casein kinase (CK) II substrate and is not phosphorylated by casein kinase I. In the presence of 150 mM NaCl, CK2 has linear kinetics for the native substrate RRRDDDSDDD with an apparent Km of 60 μM. CK2 displayed biphasic kinetics for the peptide substrate when assayed in the absence of added NaCl.

Technical Data:

M.Wt: 1706.7

Sequence (one-letter code): 5 - TMR - RRADDSDDDDD

Sequence (three-letter code): 5 - TMR - Arg - Arg - Ala - Asp - Asp - Ser - Asp - Asp - Asp - Asp - Asp - OH

Excitation (nm): 547

Emission (nm): 573

Solubility: Soluble in DMSO

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store and desiccate at -20°C and protect from light.

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References for 5 - TMR - RRADDSDDDDD :

1. Humrich, J. et al. J. Biol. Chem. 278, 4474 (2003).

2. Tiganis, T. et al. Arch. Biochem. Biophys. 325, 289 (1996).

3. Marin, O. et al. Biochem. Biophys. Res. Commun. 198, 898 (1994).

Certificate of Analysis/MSDS is available upon request

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