Calu3 cells
Catalog #:
C0016001
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Disease:
Lung carcinoma
Derived from metastatic pleural effusion
Homo sapiens
Lung
Epithelial
Adherent
The stemline chromosome number is hypotriploid
Male, Caucasian, 25 yrs of age
AddexBio-formulated EMEM (C0005-01) + 10% FBS
1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: Calu3 (AddexBio C0016001)
Origin:
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
I
Sterility:
Pathogens:
Protein Expression:
Isoenzymes:
AK-1, 1;
ES-D, 1;
G6PD, B;
GLO-I, 1-2;
Me-2, 1;
PGM1, 1;
PGM3, 1-2
DNA Profile (STR):
Amelogenin: X;
CSF1PO: 11,12;
D13S317: 12;
D16S539: 12,14;
D5S818: 11;
D7S820: 10,11;
THO1: 6,9.3 ;
TPOX: 8;
vWA: 16,17
Related cells by Organ:
References for Calu3 cells:
1. Borot, F., Vieu, D. L., Faure, G., Fritsch, J., Colas, J., Moriceau, S., Baudouin-Legros, M., Brouillard, F., Ayala-Sanmartin, J., Touqui, L., Chanson, M., Edelman, A., and Ollero, M. Eicosanoid release is increased by membrane destabilization and CFTR inhibition in Calu-3 cells. PLoS One, 4: e7116, 2009.
2. Brillault, J., De Castro, W. V., and Couet, W. Relative contributions of active mediated transport and passive diffusion of fluoroquinolones with various lipophilicities in a Calu-3 lung epithelial cell model. Antimicrob Agents Chemother, 54: 543-545.
3. Paturi, D. K., Kwatra, D., Ananthula, H. K., Pal, D., and Mitra, A. K. Identification and functional characterization of breast cancer resistance protein in human bronchial epithelial cells (Calu-3). Int J Pharm, 384: 32-38.
4. Harron, S. A., Clarke, C. M., Jones, C. L., Babin-Muise, D., and Cowley, E. A. Volume regulation in the human airway epithelial cell line Calu-3. Can J Physiol Pharmacol, 87: 337-346, 2009.
5. Alfaro-Moreno, E., Torres, V., Miranda, J., Martinez, L., Garcia-Cuellar, C., Nawrot, T. S., Vanaudenaerde, B., Hoet, P., Ramirez-Lopez, P., Rosas, I., Nemery, B., and Osornio-Vargas, A. R. Induction of IL-6 and inhibition of IL-8 secretion in the human airway cell line Calu-3 by urban particulate matter collected with a modified method of PM sampling. Environ Res, 109: 528-535, 2009.
6. Brillault, J., De Castro, W. V., Harnois, T., Kitzis, A., Olivier, J. C., and Couet, W. P-glycoprotein-mediated transport of moxifloxacin in a Calu-3 lung epithelial cell model. Antimicrob Agents Chemother, 53: 1457-1462, 2009.
