H1650 [NCI-H1650] cells
Catalog #:
C0016015
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| Frozen vial | USD450.0 |
Disease:
Stage 3B,adenocarcinoma; bronchoalveolar carcinoma
Origin:
The H1650 cell line was derived from the metastatic site of pleural effusion from a smoker.
Species:
Homo sapiens, human
Lung
Epithelial
Adherent
Not specified
Male, Caucasian, 27 years of age smoker.
AddexBio-formulated RPMI-1640 Medium(C0004-01)
+ 10% FBS
Split sub-confluent cultures (70-80%) at a seed ratio of 1:4 to 1:6. i.e. seeding at 5x10,000 cells/cm^2 using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37°C. Renew medium once every 2 to 3 days.
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,9
THO1: 9.3
TPOX: 11
vWA: 18
If use of this culture results in a scientific publication, it should be cited in the publication as: H1650 (AddexBio C0016015)
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References for H1650 cells:
1. Sordella R, Bell DW, Haber DA, Settleman J. Gefitinib-sensitizing EGFR mutations in lung cancer activate anti-apoptotic pathways. Science. 2004;305(5687):1163-1167. PMID: 15284455
