BT-483 cells
Catalog #:
C0006013
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Disease:
Ductal Carcinoma
Origin:
This cell line was isolated from a papillary invasive ductal tumor obtained from a 23 year old, normal, menstruating, parous female patient with family history of breast cancer..
Species:
Homo sapiens
Mammary gland/breast
Epithelial
Adherent
modal number = 72; range = 46 to 130
Female, 23 yrs of age, Caucasian
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS + 0.01 mg/mL bovine insulin
Subculture at 1:2; feed cells 2 to 3 times weekly. Culture at 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X,X
CSF1PO: 9,12
D13S317: 8,12
D16S539: 11,12
D5S818: 11
D7S820: 9,10
THO1: 5,9.3
TPOX: 8,11
vWA: 17
If use of this culture results in a scientific publication, it should be cited in the publication as: BT-483 (AddexBio C0006013)
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References for BT-483 cells:
1. Lasfargues EY, Coutinho WG, Redfield ES. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J Natl Cancer Inst. 1978 Oct;61(4):967-78. PMID: 212572