Disease:

Human colorectal adenocarcinoma

Origin:

This line was established from a grade IV ulcerating tumour encircling the bowel of an 82 year old Caucasian female. HLA = A32, A33, B27, B35.

Species:

Homo sapiens

Tissue:

Colon

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

2n = 46

Patient:

Female, Caucasian, 82 yrs of age

Medium:

AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% Fetal Bovine Serum (FBS).

Subculture:

1:3 to 1:6 (80-90%) using 0.25% trypsin or trypsin/EDTA, 0% CO2; 37 °C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level:

I

Sterility: