A-172 cells
Catalog #:
C0005009
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Disease:
Glioblastoma
Origin:
Derived from a glioblastoma removed from a 53 year old male. Non-tumourigenic in anti-thymocyte serum treated NIH Swiss mice.
Species:
Homo sapiens, human
Brain
Epithelial-like with processes and/or rounded
Adherent
n = 80, over 20 marker chromosomes per cell (hypertriploid)
Male, 53 years of age
AddexBio-formulated DMEM (C0003-01) + 10% FBS
Subculture sub-confluent cultures (70-80%) at 1:3 to 1:8 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.
Complete growth culture medium supplemented with 7.5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Amelogenin: X,Y
CSF1PO: 9,12
D13S317: 11
D16S539: 12
D5S818:11,12
D7S820: 11
THO1: 6, 9.3
TPOX: 8,11
vWA: 20
If use of this culture results in a scientific publication, it should be cited in the publication as: A-172 (AddexBio C0005009)
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Citation Format:
References for A-172 cells:
1. Giard DJ, Aaronson SA, Todaro GJ, Arnstein P, Kersey JH, Dosik H, Parks WP. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst. 1973 Nov;51(5):1417-1423. PMID: 4357758