MRC-5 cells
Catalog #:
T0016002
Shipping advice:
Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for details
| Frozen vial | USD420.0 |
Disease:
Normal
Origin:
The MRC-5 cell line was derived from normal lung tissue of a 14-week-old male fetus in 1966.
Species:
Human, Homo sapiens
Lung
Fibroblast
Adherent
Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal. Cytogenetic instability has been reported in the literature for some cell lines.
Male, Caucasian, 14 weeks gestation
AddexBio-formulated EMEM (C0005-01)
+ 10% FBS
Split sub-confluent cultures (70-80%) 1:2 to 1:5 using 0.25% trypsin/EDTA. Culture at 5% CO2, 37°C. Feed cells once every 2 to 3 days.
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9,11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15
If use of this culture results in a scientific publication, it should be cited in the publication as: MRC-5 (AddexBio T0016002)
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
Sterility:
Pathogens:
DNA Profile (STR):
Related cells by Organ:
References for MRC-5 cells:
1. Jacobs JP, Jones CM, Baille JP. Characteristics of a human diploid cell designated MRC-5. Nature. 1970 Jul 11;227(5254):168-170. PMID: 4316953
