THLE-2 cells
Catalog #:
T0015001
Shipping advice:
Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for details
| Frozen vial | USD420.0 |
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Disease:
Normal, SV40 immortalized, Non-tumorigenic in athymic nude mice.
Origin:
THLE-2 cell line was derived from primary normal liver cells by infection with SV40 large T antigen. -
Species:
Homo sapiens
Liver, left lobe
Epithelial;
Adherent
Near diploid
Adult
BEGM + additives without anti-fungal reagents
When confluent using 0.05% trypsin or trypsin/EDTA, subculture at 1:2 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37°C. Flask pre-coating information please refer to datasheet.
Complete culture medium supplemented with 5% (v/v) DMSO II
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 8,12
D16S539: 11,13
D5S818: 11,13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 16,17
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
Sterility:
Pathogens:
DNA Profile (STR):
References for THLE-2 cells:
1. Pfeifer AM , Cole KE, Smoot DT, Weston A, Groopman JD, Shields PG, Vignaud JM, Juillerat M, Lipsky MM, Trump BF, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5123-5127. PMID: 7685115
