3D4/2 cells
Catalog #:
T0016001
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Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for details
| Frozen vial | USD420.0 |
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Disease:
Normal macrophage; SV40 Immortalized
Origin:
The parental porcine monomyeloid cell line, 3D4, was established in December of 1998 following transfection of primary porcine alveolar macrophage cultures with the pSV3neo plasmid.
Species:
Sus scrofa, pig Lung
Macrophage
Adherent
Not available
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
When reaching 2 x 10^5 cells/cm^2 using 0.25% trypsin or trypsin/EDTA, subculture at 1:6 to 1:8; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37°C. Start culture at 5 x 10^3 cells/cm2.
Complete culture medium supplemented with 5% (v/v) DMSO II
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: 3D4/2 (AddexBio T0016001)
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References for 3D4/2 cells:
1. Weingartl HM, Sabara M, Pasick J, van Moorlehem E, Babiuk L. Continuous porcine cell lines developed from alveolar macrophages: partial characterization and virus susceptibility. J Virol Methods. 2002 Jul;104(2):203-216. PMID: 12088830
