SK-MES-1 cells
Catalog #:
C0016029
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Frozen vial | USD420.0 |
Disease:
Squamous cell carcinoma
Origin:
Derived from Metastatic Site: pleural effusion.
Species:
Homo sapiens, human
Lung
Epithelial
Adherent
The stemline chromosome number is hypotriploid, with the 2S component occurring at 3.2%. Seventeen to 20 marker chromosomes were common to most S metaphases. Normal X, 13, and 19 chromosomes were absent, and chromosomes 2, 3, 14, 17 and 20 were generally monosomic. The Y chromosome was not detected using QM staining.
Caucasian, male, 65 years of age.
AddexBio-formulated EMEM (C0005-01)
+ 10% FBS
Subculture at a ratio of 1:3 to 1:6 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.
Complete growth culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 13
D5S818: 11
D7S820: 8
THO1:6,9.3
TPOX: 8
vWA: 14
If use of this culture results in a scientific publication, it should be cited in the publication as: SK-MES-1 (AddexBio C0016029)
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Citation Format:
References for SK-MES-1 cells:
1. Fogh J, Wright WC, Loveless JD. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst. 1977;58(2):209-214. PMID: 833871