A9 cells

Catalog #:

P0011007

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Disease:

Normal

Origin:

Derivative of L929 selected in azaguanine (8-azaguanine (0.003 mg/mL) and 6-thioguanine (0.1 mM)). The cells are sensitive to HAT selection medium and deficient in adenosine phosphoribosyl transferase (APRT) and hypoxanthine phosphoribosyl transferase (HPRT). A9 may be heterozygous for the ability to synthesise active inosinic acid phosphorylase.

Species:

Mus musculus, mouse

Tissue:

Subcutaneous connective tissue; areolar and adipose

Morphology:

Fibroblast

Properties:

Adherent

Cytogenic data:

Aneuploid

Patient:

Male, C3H/An mouse, 100 days

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

Split sub-confluent cultures (70-80%) at a seed ratio of 1:3 to 1:10, i.e. seeding at 1-5x10,000 cells/cm^2 is recommended, using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37°C. Renew medium once every 2 to 3 days.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO