HEC-59 cells
Catalog #:
C0026001
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Disease:
Endometrial Carcinoma G2
Derived from endometrial carcinoma
Homo sapiens
Uterus
Epithelial
Adherent
Tumorigenic
Female, Asian
IMDM + 10% FBS
1:2 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Origin:
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
I
Sterility:
Pathogens:
Isoenzymes:
G6PD(typeB), NP, LDH
DNA Profile (STR):
D5S818:10,11,12;
D13S317:9,10,11;
D7S820:10,11,14;
D16S539:11,12,13;
VWA:17,20,22;
TH01:7,9.3;
Amelogenin:X;
TPOX:11,14;
CSF1PO:9,10
Related cells by Organ:
References for HEC-59 cells:
1. Hiramatsu, H. P., Kikuchi, Y., Seto, H., and Nagata, I. In vitro sensitivity of human endometrial cancer cell lines HEC-59 to paclitaxel or irinotecan (CPT-11) in combination with other aniticancer drugs. Anticancer Drugs, 11: 573-578, 2000.
2. Suzuki, T., Sasano, H., Sasaki, H., Fukaya, T., and Nagura, H. Quantitation of P450 aromatase immunoreactivity in human ovary HEC-59 during the menstrual cycle: relationship between the enzyme activity and immunointensity. J Histochem Cytochem, 42: 1565-1573, 1994.
3. Sasaki, H. [Aromatase activities of endometrial carcinomas HEC-59 and both basic and clinical analyses of endometrial hyperplasia as a premalignant disease]. Nihon Sanka Fujinka Gakkai Zasshi, 45: 763-775, 1993.
4. Tada, A., Sasaki, H., Nakamura, J., Yoshihama, M., and Terashima, Y. Aromatase activity and the effect of estradiol and testosterone on DNA synthesis in endometrial carcinoma cell lines HEC-59. J Steroid Biochem Mol Biol, 44: 661-666, 1993.
5. Yamamoto, T., Kitawaki, J., Urabe, M., Honjo, H., Tamura, T., Noguchi, T., Okada, H., Sasaki, H., Tada, A., Terashima, Y., and et al. Estrogen productivity of endometrium and endometrial cancer tissue; influence of aromatase on proliferation of endometrial cancer cells HEC-59. J Steroid Biochem Mol Biol, 44: 463-468, 1993.
6. Sasaki, H., Mitsunaga, S., Kitamura, T., Kishino, Y., Toyoizumi, T., Ogawa, M., Yamauchi, S., Iwata, M., Terashima, Y., and Mochizuki, S. [Radioresistant elements in adenocarcinoma of the uterus HEC-59]. Nihon Sanka Fujinka Gakkai Zasshi, 38: 1587-1594, 1986.