Hep-2C cells
Catalog #:
C0008008
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| Frozen vial | USD420.0 |
Origin:
Cervix carcinoma
Origin:
Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination. This line was originally thought to be derived from an epidermoid carcinoma of the larynx, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. The cells are positive for keratin by immunoperoxidase staining.
Species:
Homo sapiens, human
HeLa derivative, human cervix carcinoma
Epithelial
Adherent
Modal no. 76
Female, Black
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture sub-confluent cultures (85%) at 1:3 to 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.
Complete growth culture medium supplemented with 7.5% (v/v) DMSO II
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
HPV: Negative
EBV: Negative
CMV: Negative
Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818:11,12
D7S820: 8,12
THO1:7
TPOX: 8,12
vWA: 16,18
If use of this culture results in a scientific publication, it should be cited in the publication as: Hep-2C (AddexBio C0008008)
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Citation Format:
References for Hep-2C cells:
1. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PMID: 833871
