ERK 1/2 /MAP Kinase Substrate - (5-FAM) labeled

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Synonyms: 5-FAM-IPTSPITTTYFFFKKK

Functional Activity:

This is a mitogen-activated protein (MAP) kinase substrate, also designated as peptide 4. This peptide is labeled with FAM at the N-terminus. It may be used to determine activation of p38, a member of MAP kinase family which acts as a switch for the transcriptional and translational regulation of a number of proteins, including the proinflammatory cytokines. IPTSPITTTYFFFKKK is also known as an ERK1/2 substrate.

Technical Data:

M.Wt: 2277.6

Sequence (one-letter code): 5-FAM-IPTSPITTTYFFFKKK

Sequence (three-letter code): 5 - FAM - Ile - Pro - Thr - Ser - Pro - Ile - Thr - Thr - Thr - Tyr - Phe - Phe - Phe - Lys - Lys - Lys - OH

Excitation (nm): 494

Emission (nm): 521

Solubility: Soluble in DMSO

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store and desiccate at -20°C and protect from light.

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References for ERK 1/2 /MAP Kinase Substrate - (5-FAM) labeled :

1. Chen, G. et al. Biochem. 39, 2079 (2000).

Certificate of Analysis/MSDS is available upon request

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