C2C12 cells
Catalog #:
P0028001Shipping advice:
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Origin:
Derived from normal mouse leg muscleSpecies:
Mus musculus, C3HTissue:
MuscleMorphology:
MyoblastProperties:
AdherentDescription:
Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.Medium:
AddexBio-formulated DMEM (C0003-01)+ 10% FBS. Do not allow cells to reach confluence.Subculture:
1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree CFreezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSOBiosafety Level:
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Ectromelia virus (mousepox): Negative
Sterility:
Pathogens:
References for C2C12 cells:
1. Yaffe D, Saxel O. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature 270: 725-727, 1977.
2. Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997.