M-NFS-60 cells
Catalog #:
C0003022
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| Frozen vial | USD420.0 |
Disease:
Myelogenous leukemia
Origin:
The M-NFS-60 cell line was derived from a myelogenous leukemia induced with the Cas-Br-MuLV wild mouse ecotropic retrovirus. The cells are responsive to both interleukin 3 (interleukin-3, IL-3) and macrophage colony stimulating factor (M-CSF). The cells contain a truncated c-myb proto - oncogene caused by integration of a retrovirus. This cell line is a suitable transfection host.
Species:
Mus musculus, mouse
Lymphoblast
Suspension
AddexBio-formulated RPMI-1640 Medium(C0004-01)
, or equivalent medium
2-mercaptoethanol (0.05 mM) + human recombinant macrophage colony stimulating factor (M-CSF)(70 ng/mL) + 10% FBS
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2.5 X 10^4 viable cells/mL. Culture at 5% CO2, 37°C.
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: M-NFS-60
(AddexBio C0003022)
Morphology:
Properties:
Medium:
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Citation Format:
References for M-NFS-60 cells:
1. Nakoinz I, Lee MT, Weaver JF, Ralph P. Differentiation of the IL-3-dependent NFS-60 cell line and adaption to growth in macrophage colony-stimulating factor. J Immunol. 1990 Aug 1;145(3):860-864. PMID: 2142710
