THP-1 cells
Catalog #:
C0003024
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| Frozen vial | USD420.0 |
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Disease:
Acute monocytic leukemia
Origin:
This cell line was established from the peripheral blood of a 1 year old human male with acute monocytic leukemia.
Species:
Homo sapiens, human
Peripheral blood
Monocyte
Suspension
Not specified
Male, 1 years of age infant
AddexBio-formulated RPMI-1640 Medium (C0004-01)
+ 10% FBS + 2-mercaptoethanol (0.05 mM)
Cultures can be maintained by the addition or replacement of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2-4 x 10^5 viable cells/mL. Subculture when cell concentration reaches 8x10^5 cells/mL. Do not allow the cell concentration to exceed 1 x 10^6 cells/mL. Culture at 5% CO2, 37°C. Renew medium once every 2 to 3 days.
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 13
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 8,9.3
TPOX: 8,11
vWA: 16
If use of this culture results in a scientific publication, it should be cited in the publication as: THP-1 (AddexBio C0003024)
Tissue:
Morphology:
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Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
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DNA Profile (STR):
Citation Format:
References for THP-1 cells:
1. Tsuchiya S, Kobayashi Y, Goto Y, Okumura H, Nakae S, Konno T, Tada K. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 1982;42(4):1530-1536. PMID: 6949641
