L-WRN cells
Catalog #:
S0011002
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| Frozen vial | USD420.0 |
Origin:
The L-WRN cells were derived by transfecting L-Wnt3A (S0011001) with an R-spondin 3 and noggin co-expressing vector and stable clones were selected in medium containing G418 and Hygromycin B. This cell line is a source for producing Wnt-3A, R-spondin 3, and noggin conditioned medium which can be used for growing various mammalian tissue stem cells.
Species:
Mouse, Mus musculus
Subcutaneous connective tissue; areolar and adipose
Fibroblast
Adherent
Male, C3H/An, 100 days
AddexBio-formulated DMEM (C0003-01), or equivalent medium
+ 0.5 mg/mL G-418 + 0.5 mg/mL hygromycin B + 10% FBS (Wnt-3A, R-spondin 3, and noggin Conditioned Medium protocol please refer to datasheet)
When 80% confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:10; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37°C. Wnt-3A, R-spondin 3, and noggin Conditioned Medium protocol please refer to datasheet.
Complete culture medium supplemented with 10% (v/v) DMSO II
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: L-WRN (AddexBio S0011002)
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Citation Format:
References for L-WRN cells:
1. Miyoshi H, Stappenbeck TS. In vitro expansion and genetic modification of gastrointestinal stem cells in spheroid culture. Nat Protoc. 2013 Dec;8(12):2471-2482. PMID: 24232249
