HCT15 cells
Catalog #:
C0009010
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Disease:
Colorectal adenocarcinoma
Dukes' type C
Homo sapiens
Colon
Epithelial
Adherent
A quasidiploid human cell line
Male, adult
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: HCT-15 (AddexBio C0009010)
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Cytogenic data:
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Freezing Medium:
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Isoenzymes:
ES-D, 2; G6PD, B; PEP-D, 1; PGD, A; PGM1, 1-2; PGM3, 1;
DNA Profile (STR):
Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19
Citation Format:
Related cells by Organ:
References for HCT15 cells:
1. Chen TR, et al. DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin. Cancer Genet. Cytogenet. 81: 103-108, 1995.