HCC4006 cells
Catalog #:
C0016012
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Frozen vial | USD420.0 |
Disease:
Adenocarcinoma
Origin:
This cell line was derived from metastatic site: pleural effusion
Species:
Homo sapiens
Lung, Pleural effusion
Epithelial
Adherent
Not available
50+ year-old Caucasian male
RPMI-1640 Medium + 10% FBS
When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37°C. Start culture at 6 x 10^3 cells/cm2. Maintain culture at 3 x 10^4 cells/cm2.
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 11,12
D5S818: 12
D7S820: 9,12
THO1: 7
TPOX: 8,9
vWA: 16,17
If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012)
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References for HCC4006 cells:
1. Shigematsu H, Takahashi T, Nomura M, Majmudar K, Suzuki M, Lee H, Wistuba II, Fong KM, Toyooka S, Shimizu N, Fujisawa T, Minna JD, Gazdar AF. Somatic mutations of the HER2 kinase domain in lung adenocarcinomas. Cancer Res. 2005 Mar 1;65(5):1642-1646. PMID: 15753357