SVG p12 cells
Catalog #:
T0005008
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| Frozen vial | USD420.0 |
Disease:
Normal (Immortalized)
Origin:
The SVG p12 cell line was established by transfecting cultured human fetal glial cells from brain material dissected from 8 to 12 week old embryos with DNA from an ori - mutant of SV40.
Species:
Homo sapiens, human
Brain
Fibroblast; SV40 transformed astroglia
Adherent
Not specified
fetus, first trimester
AddexBio-formulated EMEM (C0005-01)
+ 10% FBS
Split sub-confluent cultures (70-80%) at a seed ratio of 1:2, i.e. seeding at 5x10,000 cells/cm^2 using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37°C. Renew medium once every 2 to 3 days.
Complete culture medium supplemented with 5% (v/v) DMSO II [Cells contain polyomavirus DNA sequences]
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Amelogenin: X,Y
CSF1PO: 10,11,12
D13S317: 8,13,14
D16S539: 10,11,12
D5S818: 11,12,14
D7S820: 10,12,13
THO1: 6,7,9,9.3
TPOX: 8,10,11
vWA: 15,16,18,19
If use of this culture results in a scientific publication, it should be cited in the publication as: SVG p12 (AddexBio T0005008)
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Citation Format:
References for SVG p12 cells:
1. Henriksen S, Tylden GD, Dumoulin A, Sharma BN, Hirsch HH, Rinaldo CH. The human fetal glial cell line SVG p12 contains infectious BK polyomavirus. J Virol. 2014;88(13):7556-7568. PMID: 24760884
