LS-180 cells
Catalog #:
C0009006
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| Frozen vial | USD420.0 |
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Disease:
Colorectal adenocarcinoma
Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon
Homo sapiens
Colon
Epithelial
Adherent
Tumorigenic, Modal no. 45 (2n=46)
Female, Caucasian, 58 yrs of age
AddexBio-formulated EMEM (C0005-01) + 10% FBS
1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: LS-180 (AddexBio C0009006)
Origin:
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
I
Sterility:
Pathogens:
Protein Expression:
HLA A2, B13, B50, IL6, IL10, mucin
Isoenzymes:
ADA, 1;
ES-D, 1;
G6PD, B;
PEP-D, 1;
PGD, A;
PGM1, 1;
PGM3, 2
DNA Profile (STR):
Amelogenin: X ;
CSF1PO: 10,13;
D13S317: 10;
D16S539: 11,13;
D5S818: 10,15;
D7S820: 11,9.3;
THO1: 6,7;
TPOX: 8,9;
vWA: 15,18
Citation Format:
Related cells by Organ:
References for LS-180 cells:
1. Bass, S. E., Sienkiewicz, P., Macdonald, C. J., Cheng, R. Y., Sparatore, A., Del Soldato, P., Roberts, D. D., Moody, T. W., Wink, D. A., and Yeh, G. C. Novel dithiolethione-modified nonsteroidal anti-inflammatory drugs in human hepatoma HepG2 and colon LS180 cells. Clin Cancer Res, 15: 1964-1972, 2009.
2. Fukumori, S., Masago, M., Ishida, K., Kayano, Y., Taguchi, M., and Hashimoto, Y. Temperature-dependent specific transport of levofloxacin in human intestinal epithelial LS180 cells. Biopharm Drug Dispos, 30: 448-456, 2009.
3. Graber-Maier, A., Buter, K. B., Aeschlimann, J., Bittel, C., Kreuter, M., Drewe, J., and Gutmann, H. Effects of Curcuma extracts and curcuminoids on expression of P-glycoprotein and cytochrome P450 3A4 in the intestinal cell culture model LS180. Planta Med, 76: 1866-1870.
4. Ishida, K., Takaai, M., Yotsutani, A., Taguchi, M., and Hashimoto, Y. Membrane transport mechanisms of mizoribine in the rat intestine and human epithelial LS180 cells. Biol Pharm Bull, 32: 741-745, 2009.
5. Takaai, M., Morishita, H., Ishida, K., Taguchi, M., and Hashimoto, Y. Contribution of Na+-independent nucleoside transport to ribavirin uptake in the rat intestine and human epithelial LS180 cells. Eur J Pharmacol, 601: 61-65, 2008.
6. Yamasaki, D., Nakamura, T., Okamura, N., Kokudai, M., Inui, N., Takeuchi, K., Watanabe, H., Hirai, M., Okumura, K., and Sakaeda, T. Effects of acid and lactone forms of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors on the induction of MDR1 expression and function in LS180 cells. Eur J Pharm Sci, 37: 126-132, 2009.
