INS-1 832/13 cells
Catalog #:
C0018024
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| Frozen vial | USD560.0 |
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Disease:
Insulinoma
Origin:
INS-1 832/13 is a derivative of INS-1 (C0018007) cells originally established from an x-ray induced insulinoma in rat. The INS-1 832/13 cell line is a subclone of INS-1 that was stably transfected with a CMV promoter-human ins expression plasmid carrying a geneticin (G418)-resistance marker for selection.
Species:
Rattus Norvegicus
Pancreas
Beta cells
Adherent
AddexBio-Optimized RPMI-1640 Medium(C0004-02) + 10% FBS + 0.05 mM 2-mercaptoethanol
Subculture sub-confluent cultures (85%) at 1:5 to 1:6 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 10% CO2, 37°C.
Complete growth culture medium supplemented with 7.5% (v/v) DMSO II
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
HPV: Negative
EBV: Negative
CMV: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: INS-1 832/13 (AddexBio C0018024)
Tissue:
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Citation Format:
References for INS-1 832/13 cells:
1. Hohmeier HE, Mulder H, Chen G, Henkel-Rieger R, Prentki M, Newgard CB. Isolation of INS-1-derived cell lines with robutst ATP-sensitive K+ channel-dependent and -independent glucose-stimulated ins secretion. Diabetes, 2000;49(3):424-430. PMID: 10868964
