BT-20 cells

Catalog #:

C0006014

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Disease:

Carcinoma

Origin:

This breast tumor line was established in 1958 by isolation and cultivation of cells spilling out of the tumor when it was cut in thin slices. ¬

Species:

Homo sapiens

Tissue:

Mammary gland/breast

Morphology:

Epithelial;

Properties:

Adherent

Cytogenic data:

Normal chromosomes N3, N4, N9, N13, N14, and X may be absent. The markers der(11)t(11;?)(q25;?) (M1); der(1)t(1;3)(p22?;p13?) (M2); and der(2)t(2;?) (q37;?) (M5) were detected

Patient:

74 year old Caucasian, female

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Subculture at 1:2 to 1:4; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37°C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO