MDA-MB-468 cells
Catalog #:
C0006003
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Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for details
| Frozen vial | USD420.0 |
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Disease:
Adenocarcinoma
Origin:
Established in 1977 from the pleural effusion of a 51-year-old black woman with metastatic adenocarcinoma of the breast; these cells carry a p53 point mutation at codon 273 leading to Arg-His substitution
Species:
Homo sapiens
Breast, mammary glands
Epithelial
Adherent
modal number = 64; range = 60 to 67.
Female, Black, 51 yrs of age
AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBS
Seed cells at 1-2 x 10^6 cells in T75 flask; split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA; without CO2 (or use non-vented flask and keep the lid closed); 37°C
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 9
D5S818: 12
D7S820: 8
THO1: 7
TPOX: 8,9
vWA: 18
If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003)
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
Sterility:
Pathogens:
Isoenzymes:
AK-1, 1;
ES-D, 1;
G6PD, A;
GLO-I, 1-2;
Me-2, 1-2;
PGM1, 1;
PGM3, 2
DNA Profile (STR):
Citation Format:
Related cells by Organ:
References for MDA-MB-468 cells:
1. Cailleau, R., Olive, M., Cruciger, Q. V. (1978). Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14 ( 11 ): 911-915. http://www.ncbi.nlm.nih.gov/pubmed/730202
2. Nigro, J. M., Baker, S. J., Preisinger, A. C., Jessup, J. M., Hostetter, R., Cleary, K., Bigner, S. H., Davidson, N., Baylin, S., Devilee, P., . (1989). Mutations in the p53 gene occur in diverse human tumour types. Nature 342 ( 6250 ): 705-708. http://www.ncbi.nlm.nih.gov/pubmed/2531845
