SK-BR-3 cells
Catalog #:
C0006007
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| Frozen vial | USD420.0 |
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Disease:
Breast adenocarcinoma
Pleural effusion
Homo sapiens
Breast; mammary gland
Epithelial
Adherent
This is a hypertriploid to hypotetraploid human cell line(+A, +B, +C, +E, +F, +G, -D) with abnormalities including dicentrics, acrocentric fragments, rings, secondary constrictions, large metacentrics or polycentrics and large submetacentric marker
Female, Caucasian, 43 yrs of age
McCoy's 5a (Gibco 16600082) + 10% FBS
1:2 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C
Complete culture medium supplemented with 5% (v/v) DMSO
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X
CSF1PO: 12
D13S317: 11,12
D16S539: 9
D5S818: 9,12
D7S820: 9,12
THO1: 8,9
TPOX: 8,11
vWA: 17
If use of this culture results in a scientific publication, it should be cited in the publication as: SK-BR-3 (AddexBio C0006007)
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Protein Expression:
HER2/c-erb-2
Isoenzymes:
AK-1, 1-2;
ES-D, 1;
G6PD, B;
GLO-I, 2;
PGM1, 1-2;
PGM3, 1
DNA Profile (STR):
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References for SK-BR-3 cells:
1. Brockhoff, G., Heckel, B., Schmidt-Bruecken, E., Plander, M., Hofstaedter, F., Vollmann, A., and Diermeier, S. Differential impact of Cetuximab, Pertuzumab and Trastuzumab on BT474 and SK-BR-3 breast cancer cell proliferation. Cell Prolif, 40: 488-507, 2007.
2. Choi, E. J. and Kim, G. H. Apigenin causes G(2)/M arrest associated with the modulation of p21(Cip1) and Cdc2 and activates p53-dependent apoptosis pathway in human breast cancer SK-BR-3 cells. J Nutr Biochem, 20: 285-290, 2009.
3. Hill, A. A., LaPan, P., Li, Y., and Haney, S. Impact of image segmentation on high-content screening data quality for SK-BR-3 cells. BMC Bioinformatics, 8: 340, 2007.
4. Koster, F., Felberbaum, R., Finas, D., Wunsch, K., Schulz, C., Diedrich, K., and Hauser, C. Progesterone and estradiol enhance lipid mediated transfection of Sk-Br-3 mammalian cancer cells. Int J Mol Med, 9: 617-620, 2002.
5. Lin, Y. J., Huang, Y. H., Zhen, Y. Z., Liu, X. J., and Zhen, Y. S. [Rhein lysinate induces apoptosis in breast cancer SK-Br-3 cells by inhibiting HER-2 signal pathway]. Yao Xue Xue Bao, 43: 1099-1105, 2008.
6. Manfroid, I., Van de Weerdt, C., Baudhuin, A., Martial, J. A., and Muller, M. EGF stimulates Pit-1 independent transcription of the human prolactin pituitary promoter in human breast cancer SK-BR-3 cells through its proximal AP-1 response element. Mol Cell Endocrinol, 229: 127-139, 2005.
