5 - FAM - GRPRTSSFAEG (GSK-3 peptide analog)

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Synonyms: Crosstide [GRPRTSSFAEG], 5 - FAM labeled

Functional Activity:

Crosstide is a peptide analog of glycogen synthase kinase-3 (GSK-3) that functions as a natural substrate for Akt/PKB. It displays similar specificities towards PKBα, PKBβ and PKBγ isoforms. It is also recognized by MAPKAP kinase-1 and p70S6K. The fluorescent and biotinlyated peptides demonstrate similar enzyme activities.

Technical Data:

M.Wt: 1522.6

Sequence (one-letter code): 5-FAM-GRPRTSSFAEG

Sequence (three-letter code): 5 - FAM - Gly - Arg - Pro - Arg - Thr - Ser - Ser - Phe - Ala - Glu - Gly - OH

Excitation (nm): 494

Emission (nm): 521

Solubility: Soluble in DMSO

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store and desiccate at -20°C and protect from light.

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References for 5 - FAM - GRPRTSSFAEG :

1. Dai, F. et al. Biochem. Biophys. Res. Commun. 293, 1191 (2002).

2. Meier, R. et al. J. Biol. Chem. 272, 30491 (1997); Chan, TO. et al. Ann. Rev. Biochem. 68, 965 (1999).

Certificate of Analysis/MSDS is available upon request

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