COS-1 cells
Catalog #:
T0014001
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Origin:
Kidney fibroblasts
Cercopithecus aethiops(African Green Monkey)
Kidney
Fibroblast-like
Adherent
SV40 immortalized
DMEM + 10% FBS
1:4 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C
Complete culture medium supplemented with 5% (v/v) DMSO
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative Species:
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References for COS-1 cells:
1. Hurtado, C., Bustos, M. J., and Carrascosa, A. L. The use of COS-1 cells for studies of field and laboratory African swine fever virus samples. J Virol Methods, 164: 131-134.
2. Ndong, M., Kazami, M., Suzuki, T., Uehara, M., Katsumata, S., Inoue, H., Kobayashi, K., Tadokoro, T., Suzuki, K., and Yamamoto, Y. Iron deficiency down-regulates the Akt/TSC1-TSC2/mammalian Target of Rapamycin signaling pathway in rats and in COS-1 cells. Nutr Res, 29: 640-647, 2009.
3. Ge, K., Sun, R., and Tian, Z. Rapid immunosorting of transmembrane proteins of Lymphocytes from a cDNA expression library of COS-1 cells. Immunol Invest, 38: 589-601, 2009.
4. Miyake, Y., Tsuzuki, S., Yasumoto, M., Fushiki, T., and Inouye, K. Requirement of the activity of hepatocyte growth factor activator inhibitor type 1 for the extracellular appearance of a transmembrane serine protease matriptase in monkey kidney COS-1 cells. Cytotechnology, 2009.
5. Joubel, A., Chalkley, R. J., Medzihradszky, K. F., Hondermarck, H., and Burlingame, A. L. Identification of new p53 acetylation sites in COS-1 cells. Mol Cell Proteomics, 8: 1167-1173, 2009.
6. Smith, S. L. and Shioda, T. Advantages of COS-1 monkey kidney epithelial cells as packaging host for small-volume production of high-quality recombinant lentiviruses. J Virol Methods, 157: 47-54, 2009.
7. Gemeinhardt, A., Alfalah, M., Guck, T., Naim, H. Y., and Fuhrmann, H. The influence of linoleic and linolenic acid on the activity and intracellular localisation of phospholipase D in COS-1 cells. Biol Chem, 390: 253-258, 2009.