MDA-MB-157 cells
Catalog #:
C0006020
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| Frozen vial | USD420.0 |
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Disease:
Medulallary carcinoma
Origin:
This cell line was isolated from pleural effusions.
Species:
Homo sapiens, human
Mammary gland; breast/medulla
Epithelial
Adherent
modal number =52 to 54; range = 52 to 69.
Most of the karyotypes had three normal X chromosomes. Normal chromosomes N1, N5, N13, N14, N15, N16, N19, and N22 were not found. Seventeen marker chromosomes were identified: t(10q14q) der(12)t(1;12)(q21;p13), der(7)t(7;?)(q22;?), del(1)(q11), t(3qter>3q21::10p15>10qter), 7q+, del(5)(q13), 19q+, t(10q;14q), der(4)t(1;4)(q21;q25), 9q+, 13p+, 15p+ unknown, unknown, unknown, del(6)(q21).
Black, female, 44 years of age.
AddexBio-formulated Leibovitz's L-15 Medium (C0006-01)
+ 10% FBS
A subcultivation ratio of 1:2 to 1:3 is recommended using 0.25% trypsin or trypsin/EDTA. Culture at 100% Air, 37°C.
Complete growth culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 10,11
THO1:7,8
TPOX: 11,9
vWA: 15
If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
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Citation Format:
References for MDA-MB-157 cells:
1. Young RK, Cailleau RM, Mackay B, Reeves WJ. Establishment of epithelial cell line MDA-MB-157 from metastatic pleural effusion of human breast carcinoma. In Vitro. 1974;9(4):239-245. PMID: 4471183
