CCRF-CEM cells
Catalog #:
C0003001
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| Frozen vial | USD420.0 |
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Disease:
Acute lymphoblastic leukemia
Lymphoblast
Homo sapiens
Blood
Lymphoblast
Suspension
CCRF-CEM cells were derived from the peripheral blood buffy coat of a child (CEM) with acute lymphoblastic leukemia who had originally presented with lymphosarcoma
Female, Caucasian, 4 yrs of age
RPMI1640 + 10% FBS
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 105 viable cells/ml. Maintain cell density between 1 to 2 X 106 viable cells/ml.
Add fresh medium (20% to 30% by volume) every 2 to 3 days.
Complete culture medium supplemented with 5% (v/v) DMSO
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Origin:
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Medium Renewal:
Freezing Medium:
Biosafety Level:
I
Sterility:
Pathogens:
Isoenzymes:
ADA, 1;
ES-D, 1;
G6PD, B ;
GLO-I, 1;
PEP-D, 1;
PGD, C;
PGM1, 1 ;
PGM3, 0
DNA Profile (STR):
Amelogenin: X ;
CSF1PO: 10,11;
D13S317: 11,12;
D16S539: 10,13;
D5S818: 12,13;
D7S820: 9,13;
THO1: 6,7;
TPOX: 8;
vWA: 17,19
Related cells by Organ:
CCRF-CEM
References for CCRF-CEM cells:
1. Adams, M., Efferth, T., and Bauer, R. Activity-guided isolation of scopoletin and isoscopoletin, the inhibitory active principles towards CCRF-CEM leukaemia cells and multi-drug resistant CEM/ADR5000 cells, from Artemisia argyi. Planta Med, 72: 862-864, 2006.
2. Anderson, A. B. and Arriaga, E. A. Subcellular metabolite profiles of the parent CCRF-CEM and the derived CEM/C2 cell lines after treatment with doxorubicin. J Chromatogr B Analyt Technol Biomed Life Sci, 808: 295-302, 2004.
3. Henrich, S., Crossett, B., and Christopherson, R. I. Differentially expressed nuclear proteins in human CCRF-CEM, HL-60, MEC-1 and Raji cells correlate with cellular properties. Proteomics Clin Appl, 1: 1252-1265, 2007.
4. Jow, G. M., Wu, Y. C., Guh, J. H., and Teng, C. M. Armepavine oxalate induces cell death on CCRF-CEM leukemia cell line through an apoptotic pathway. Life Sci, 75: 549-557, 2004.
5. Kong, Y., Ma, W., Liu, X., Zu, Y., Fu, Y., Wu, N., Liang, L., Yao, L., and Efferth, T. Cytotoxic activity of curcumin towards CCRF-CEM leukemia cells and its effect on DNA damage. Molecules, 14: 5328-5338, 2009.
6. Zu, Y., Liu, X., Fu, Y., Shi, X., Wu, N., Yao, L., and Efferth, T. Cytotoxic activity of isoliquiritigenin towards CCRF-CEM leukemia cells and its effect on DNA damage. Planta Med, 75: 1134-1140, 2009.
