Alpha TC1 Clone 9 (Alpha TC1-9) cells
Catalog #:
C0018021
Shipping advice:
Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for details
| Frozen vial | USD560.0 |
Disease:
Mouse islet cell adenoma
Origin:
Alpha TC1 clone 9 is a pancreatic alpha cell line cloned from the alpha TC1 cell line which was derived from an adenoma created in transgenic mice expressing the SV40 large T antigen oncogene under the control of the rat preproglucagon promoter. This cell line is heterozygous for MHC alleles derived from both parental strains used in construction of the transgenic mice [C57BL/6J(H-2b] and DBA/2J(H-2d)].
Species:
Mus musculus, mouse
Pancreas; alpha cell
Epithelial-like
Adherent; single cells and loosely attached clusters
(C57BL/6J x DBA/2)F2 (transgenic for the SV40 T antigen)
AddexBio-specialized DMEM (C0003-03) + 0.1 mM Non-essential amino acids (NEAA) + 10% FBS
Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 10% CO2, 37°C.
Complete growth culture medium supplemented with 7.5% (v/v) DMSO II
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: Alpha TC1-9 (AddexBio C0018021)
Tissue:
Morphology:
Properties:
Strain:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
Sterility:
Citation Format:
References for Alpha TC1-9 cells:
1. Powers AC, et al. Proglucagon processing similar to normal islets in pancreatic alpha-like cell line derived from transgenic mouse tumor. Diabetes 39: 406-414, 1990. PMID: 2156740
