B16-F10 cells

Catalog #:

C0012005

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Disease:

Melanoma

Origin:

Established from a B16 melanoma tumor grown in syngeneic C57BL/6 mice.

Species:

Mus musculus, mouse

Tissue:

Skin

Morphology:

Mixture of spindle-shaped and epithelial-like cells

Properties:

Adherent

Strain:

C57BL/6J

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO