MARCKS Protein (154 - 165) - (5-TAMRA) labeled

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Synonyms: 5-TMR-KKRFSFKKSFKL

Functional Activity:

Peptide sequence KKRFSFKKSFKL is derived from amino acid residues 154 ?165 of myristoylated alanine-rich C-kinase substrate (MARCKS) protein phosphorylation site domain (PSD). It retains the first two of the four serine residues in the PSD. This peptide is proven to be a generic substrate for protein kinase C (including PKCα and β, naturally-occurring PKC δ, ε, and θ, as well as atypical PKC ξ) at Ser5 and Ser9, even though atypical PKCs do not utilize intact MARCKS as a substrate.

Technical Data:

M.Wt: 1956.4

Sequence (one-letter code): 5-TMR-KKRFSFKKSFKL

Sequence (three-letter code): 5 - TMR - Lys - Lys - Arg - Phe - Ser - Phe - Lys - Lys - Ser - Phe - Lys - Leu - OH

Excitation (nm): 547

Emission (nm): 573

Solubility: Soluble in DMSO

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store and desiccate at -20°C and protect from light.

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References for MARCKS Protein (154 - 165) - (5-TAMRA) labeled :

1. Irie, K. et al. Pharmacol. Ther. 93, 271 (2002);

2. Uberall, F. et al. J. Biol. Chem. 272, 4072 (1997);

3. Blackshear, P. J. Biol. Chem. 268, 1501 (1993).

Certificate of Analysis/MSDS is available upon request

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