Raji cells
Catalog #:
C0003009
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Frozen vial | USD420.0 |
Disease:
Burkitt's lymphoma
Origin:
Established from the left maxilla of a 12-year-old African boy with Burkitt lymphoma in 1963; first continuous human hematopoietic cell line
Homo sapiens
Lymphoblast
Lymphoblast
Round single cells in suspension, also in clusters or clumps
human flat-moded hypotetraploid karyotype with 12% polyploidy - 89(80-91)<4n>XXYY, -4, -9, -12, -16, -18, -21, +3mar, add(4)(q35), add(6)(q27)x2, t(8;14)(q24;q32), der(8)t(8;14) (q24;q32)add(8)(?p12), del(9)(p23), der(14)t(8;14)(q24;q32) - carries t(8;14) associated with Burkitt lymphoma - closely resembles depositor's karyotype
Male, Black, 11 yrs of age
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
Optimal split ratio of saturated culture at 1:4 to 1:8 twice a week; seed out at 0.5 x 10^6 cells/ml; maximum of 1.5x10^6 viable cells/mL is considered saturated culture; 5% CO2; 37 °C
Complete culture medium supplemented with 5% (v/v) DMSO I (The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1).
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
Sterility:
Pathogens:
DNA Profile (STR):
Amelogenin: X,Y;
CSF1PO: 10,12;
D13S317: 13;
D16S539: 8,11;
D5S818: 10,13;
D7S820: 10;
THO1: 6,7;
TPOX: 8,13;
vWA: 16,19
References for Raji cells:
1. Pulvertaft JV. Cytology of Burkitt's tumour (African lymphoma). Lancet 1: 238-240, 1964.