5-ROX, NHS ester

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Synonyms: 5 - Carboxy - X - rhodamine, succinimidyl ester

Functional Activity:

The isomeric 5-ROX, SE is an amine-reactive form of carboxy-X-rhodamine and is used as an amine coupling reagent to form 5-carboxy-X-rhodamine (ROX) derivatized compounds such as proteins, nucleic acids and drugs that may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications. Labeling reagent for preparation of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing.

Technical Data:

M.Wt: 631.67

Formula: C₃₇H₃₃ N₃ O₇

Excitation (nm): 573

Emission (nm): 602

Solubility: Soluble in DMSO, DMF

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store at -20°C and protect from light.

CAS No.: 209734-74-7

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References for 5-ROX, NHS ester :

1. Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J. (2005) Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides. Proc Natl Acad Sci U S A, 102, 5926.

1. Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J. (2005) Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides. Proc Natl Acad Sci U S A, 102, 5926.