5-ROX, NHS ester
Catalog #:
ABBFO1029Shipping advice:
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ABBFO1029-5mg | USD149.0 |
Synonyms: 5 - Carboxy - X - rhodamine, succinimidyl ester

Functional Activity:
The isomeric 5-ROX, SE is an amine-reactive form of carboxy-X-rhodamine and is used as an amine coupling reagent to form 5-carboxy-X-rhodamine (ROX) derivatized compounds such as proteins, nucleic acids and drugs that may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications. Labeling reagent for preparation of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing.
Technical Data:
M.Wt: 631.67
Formula: C37H33 N3 O7
Excitation (nm): 573
Emission (nm): 602
Solubility: Soluble in DMSO, DMF
Purity: >95% (HPLC)
Shipping Conditions: Room temperature
Storage: Store at -20°C and protect from light.
CAS No.: 209734-74-7
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References for 5-ROX, NHS ester :
1. Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J. (2005) Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides. Proc Natl Acad Sci U S A, 102, 5926.
1. Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J. (2005) Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides. Proc Natl Acad Sci U S A, 102, 5926.