5(6)-ROX, SE

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Synonyms: 5 - (and - 6) - Carboxy - X - rhodamine, succinimidyl ester

Functional Activity:

Mixed isomers of the succinimidyl ester of carboxy-X-rhodamine (ROX). It is amine-reactive and is the primary labeling reagents for preparing various ROX-based bioconjugates. Derivatives made with the carboxy-X-rhodamine (ROX), succinimidyl ester are widely used for oligonucleotide labeling and automated DNA sequencing applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lissamine? rhodamine B conjugate.

Technical Data:

M.Wt: 631.67

Formula: C₃₇H₃₃ N₃ O₇

Excitation (nm): 576

Emission (nm): 601

Solubility: Soluble in DMSO, DMF

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store at -20°C and protect from light.

CAS No.: 114616-32-9

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References for 5(6)-ROX, SE :

1. Bertram JG, et al. (2000). Molecular mechanism and energetics of clamp assembly in Escherichia coli. The role of ATP hydrolysis when gamma complex loads beta on DNA. J Biol Chem 275, 28413-20;

2. Rodionov VI, et al. (1994). Microtubule dynamics in fish melanophores. J Cell Biol 126, 1455-64.