5-FAM-X, SE

Catalog #:

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Synonyms: 6 - (Fluorescein - 5 - carboxamido)hexanoic acid, succinimidyl ester

Functional Activity:

5-FAM-X, SE contains a seven-atom aminohexanoyl spacer (known as 'X') between the FAM fluorophore and the succinimidyl ester. The 'X' spacer separates the fluorophore from the biomolecule to which it is conjugated. The 'X' spacer may potentially reduce the quenching that typically occurs when a fluorescein molecule is conjugated to proteins or other biopolymers (such as nucleic acids).

Technical Data:

M.Wt: 586.55

Formula: C₃₁H₂₆N₂O₁₀

Excitation (nm): 494

Emission (nm): 521

Solubility: Soluble in DMSO, DMF

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store and desiccate at -20°C and protect from light.

CAS No.: 114616-31-8

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References for 5-FAM-X, SE :

1. Czapski GA, et al. (2001). Detection of protein oxidation in endothelial cells by fluorescently labeled tyramine. Med Sci Monit 7, 606-9.

2. Cooper WC, et al. (2000). Detection of fluorescently labeled actin-bound cross-bridges in actively contracting myofibrils. Biophys J 78, 1449-57.