5-FAM SE

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Synonyms: 5 - Carboxyfluorescein, succinimidyl ester

Functional Activity:

5-FAM SE is the amine-reactive succinimidyl ester of single isomer 5-FAM acid. It is widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate such as FITC.

Technical Data:

M.Wt: 473.39

Formula: C₂₅H₁₅ NO₉

Excitation (nm): 492

Emission (nm): 518

Solubility: Soluble in DMSO, DMF

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store at -20°C and protect from light.

CAS No.: 92557-80-7

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References for 5-FAM SE :

1. Sakamoto M, et al. (2003). Application of terminal RFLP analysis to characterize oral bacterial flora in saliva of healthy subjects and patients with periodontitis. J Med Microbiol 52, 79-89.

2. Hahn M, et al. (2001). Influence of fluorophore dye labels on the migration behavior of polymerase chain reaction-amplified short tandem repeats during denaturing capillary electrophoresis. Electrophoresis 22, 2691-700.