5-TAMRA

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Synonyms: 5-Carboxytetramethylrhodamine

Functional Activity:

5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is widely used to label peptides and proteins. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein. 5-TAMRA has also achieved prominence as a dye for oligonucleotide labeling and automated DNA sequencing applications.

Technical Data:

M.Wt: 430.46

Formula: C₂₅H₂₂N₂O₅

Excitation (nm): 541

Emission (nm): 568

Solubility: Soluble in DMSO, DMF

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store at room temperature and protect from light.

CAS No.: 91809-66-4

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References for 5-TAMRA :

1. Unruh JR, Gokulrangan G, Wilson GS, Johnson CK. (2005) Fluorescence properties of fluorescein, tetramethylrhodamine and Texas Red linked to a DNA aptamer. Photochem Photobiol, 81, 682.

2. Vamosi G, Gohlke C, Clegg RM. (1996) Fluorescence characteristics of 5- carboxytetramethylrhodamine linked covalently to the 5' end of oligonucleotides: multiple conformers of single-stranded and double-stranded dye-DNA complexes. Biophys J, 71, 972.