5-CR6G

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Synonyms: 5-Carboxyrhodamine 6G

Functional Activity:

5-CR6G is a single isomer of 5(6)-CR6G. It is preferred for some complicated biological applications where reproducibility is more critical because the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates. In general, rhodamine 6G conjugates have higher fluorescence quantum yields than tetramethylrhodamine conjugates, as well as good photostability. 5-CR6G is used to modify amino and hydroxy groups using EDC-mediated couplings when there are difficulties in using 5-CR6G SE. The excitation and emission wavelengths of rhodamine 6G fall between those of fluorescein and tetramethylrhodamine derivatives, making 5-CR6G another color choice for the multicolor fluorescence imaging applications.

Technical Data:

M.Wt: 494.97

Formula: C₂₇H₂₆N₂O₅

Excitation (nm): 518

Emission (nm): 544

Solubility: Soluble in DMSO

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Desiccated at -20 °C and protect from light.

CAS No.: N/A

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References for 5-CR6G :

1. Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J. (2005) Fourcolor DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides. Proc Natl Acad Sci U S A, 102, 5926.

2. Dietrich A, Buschmann V, Muller C, Sauer M. (2002) Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data. J Biotechnol, 82, 211.