6-FAM SE

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Synonyms: 6 - Carboxyfluorescein, succinimidyl ester

Functional Activity:

6-FAM SE is the amine-reactive succinimidyl ester of single isomer 6-FAM acid. It is one of the most popular green fluorescent reagents used for labeling nucleotides and nucleic acids. Compared to 5-FAM, 6-FAM is less often used to prepare small molecules.

Technical Data:

M.Wt: 473.39

Formula: C₂₅H₁₅ NO₉

Excitation (nm): 495

Emission (nm): 517

Solubility: Soluble in DMSO, DMF

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store at -20°C and protect from light.

CAS No.: 92557-81-8

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References for 6-FAM SE :

1. Jordan JA, et al. (2001). TaqMan-based detection of Trichomonas vaginalis DNA from female genital specimens. J Clin Microbiol 39, 3819-22.

2. Brandis JW (1999). Dye structure affects Taq DNA polymerase terminator selectivity. Nucleic Acids Res 27, 1912-8.