5(6)-FAM SE

Catalog #:

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Synonyms: 5 - (and - 6) - Carboxyfluorescein, succinimidyl ester

Functional Activity:

5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate such as FITC.

Technical Data:

M.Wt: 473.39

Formula: C₂₅H₁₅ NO₉

Excitation (nm): 494

Emission (nm): 519

Solubility: Soluble in DMSO, DMF

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store at -20°C and protect from light.

CAS No.: 117548-22-8

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References for 5(6)-FAM SE :

1. Hahn M, et al. (2001). Influence of fluorophore dye labels on the migration behavior of polymerase chain reaction-amplified short tandem repeats during denaturing capillary electrophoresis. Electrophoresis 22, 2691-700

2. Sanders SJ (2000). Factor V Leiden genotyping using real-time fluorescent polymerase chain reaction. Mol Cell Probes 14, 249-53.